P-86: Expression IP-10 and CXCR3 Transcripts in Peripheral Blood of Ovarian Cancer Patients Compared to Control Cases
Objective: Ovarian cancer has the highest mortality rate among gynecologic cancers and ranks in the top five causes of cancer death among women. The high case fatality rate is partly the result of delayed diagnosis and the lack of an effective treatment for women who have advanced disease. Consequently, significance has grown for the determination of prognostic markers identifying patients to benefit more from adjuvant systemic therapies and develop predictive markers for response to different treatments. Identification of circulating tumor markers is one of recent high concern in tumor research. The IFN-γ induced protein-10 (CXCL10/IP-10) is produced by a variety of cells, including endothelial cells, fibroblasts, mononuclear cells and tumor cells and its presence has been confirmed in several types of tumor tissues. CXCR3 is known as ligand of IP-10 and involved in the chemotactic activity of the IFN-γ-induced CXC chemokines on activated T lymphocytes and NK cells. Classically, the recruitment of these cells facilitates anti-tumor immunity resulting in tumor regression. CXCR3 is postulated to mediate the angiostatic activity of IP-10 on endothelial cells. To further examine the importance role of CXCR3 and CXCL10/IP-10 in the cancer patients, we will evaluate the expression levels of CXCR3 and CXCL10/IP-10 transcripts in the peripheral blood ovarian cancer patients by Real Time PCR (RT-PCR) method in compare healthy women and also their correlation with each other will be examined. Materials and Methods: Peripheral blood specimen from 48 patients with ovarian cancer and healthy women with same age was collected. Total RNA was extracted with TRIzol reagent and cDNA was synthesized. Expression of β-actin (housekeeping gene), CXCR3 and IP-10 was evaluated using Real-Time PCR and Syber green I as reporter dye. At the final step of each run, Melting Curve analyzing was performed to confirm validity of results. Statistical analyzing was done using SPSS software by Mann-Whitney and correlation tests. Results: As a result the expression level of CXCR3 was not significantly different in patients when compared with control group. However, IP-10 expression increased significantly in ovarian cancer patients in comparison with control cases (p< 0.05). Moreover, IP-10 expression was significantly correlated with CXCR3 expression in both patient and control groups (p<0.001). Conclusion: Results of this study showed that expression level of some gene such as IP-10 can be applied in cancer diagnosis but validity and accuracy of this finding must be further examined in extended groups.