P-91: Comparative Detection of (VP1) D1 Genomes in Foot-and-Mouth Disease Virus (FMDV) Serotype A,O and Asial1 by RT-PCR


Khodaie B *, Ahamdi M , Lotfinia AA , Kamyabi Z ,

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Objective: Foot-and-mouth disease (FMD) is a highly infectious viral infection of cattle, pigs, sheep, goats, buffalo, and on artioductyle wildlife species. It is characterized by fever, vesicles in the mouth and on the muzzle, teats, and feet, and death in young animals.FMD is caused by an aphthovirus of the family picornaviridae. There are seven immunologically distinct serotypes: A, O, Asia1, and SAT (southern African territories) 1, 2, and3. Materials and Methods: Foot-and-mouth disease (FMD) is one the most important virus disease in farm animals. Type O, A, ASIA1< FMD have been endemic in Iran. In this study, samples from suspected livestock were analysed by RT-PCR experiment. The number of 970 nucleotides determined at 1D-2B region of types A, O, Asia1 strains isolated from all of the samples. The sequences have been compared with each other by Generunner software. Results: FMDV serotypes O and A whereas for serotype Asia 1, much less information Is available. The viral RNA extracted from the BHK-21 cell culture infected Supernatants was amplified by RT-PCR in the VP1 coding region to obtain PCR Products of 908/911 base pairs length The purified PCR products were sequenced Directly by cycle sequencing using the primers list. According to the to evaluation serotypes O and A had detect same variety of sequences to translating the VP1. Conclusion: The present work was undertaken to study the molecular phylogeny And evolution of Asia 1 virus isolates recovered from suspected livestock