P-131: Assessing the Role of CD271 Isolation from GCSF Mobilized Peripheral Blood in Isolation of MSCs


Attar A *, Pourhabibi Zarandi N , Ahrari I , Khosravi Maharlooi M , Monabati A ,

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Objective: CD271, also known as LNGFR (low-affinity nerve growth factor receptor), belongs to the low-affinity neurotrophin receptor and the tumor necrosis factor receptor superfamily. CD271 (LNGFR) is a well-known marker for the enrichment of mesenchymal stromal cells (MSCs). Isolated CD271+ cells from bone marrow have a higher proliferative capacity in comparison to MSCs isolated by plastic adherence. Since Isolation of MSCs from GCSF mobilized peripheral blood using regular culturing techniques usually does not lead to MSCs isolation. Reaserchers have used techniques such as fibrin microbead to isolate these cells from GCSF mobilized peripheral blood. In the present study we have addressed the question whether CD271 isolation can enhance the isolation rate of MSCs or not? Materials and Methods: 5cc of GCSF mobilized peripheral blood from the remnant part of bags used for bone marrow transplantation was collected. Cells were isolated on Ficoll layer and underwent CD271 isolation using magnetic activated cell sorting (MACS) technique. Bone marrow sample was used as the internal control. Results: CD271 positive portion of GCSF mobilized peripheral blood did not yield to any cell outgrowth but the bone marrow sample successfully led to MSC isolation. Conclusion: CD271 positive selection cannot help isolating MSCs from GCSF mobilized peripheral blood