P-132: CD271 Enrichment Is Not Useful for Enhancing the Rate of Mesenchymal Stromal Cells Isolation from Umbilical Cord Blood
Objective: CD271, also known as LNGFR (low-affinity nerve growth factor receptor), belongs to the low-affinity neurotrophin receptor and the tumor necrosis factor receptor superfamily. CD271 (LNGFR) is a well-known marker for the enrichment of mesenchymal stromal cells (MSCs). Isolated CD271+ cells from bone marrow have a higher proliferative capacity in comparison to MSCs isolated by plastic adherence. Isolation of MSCs from Umbilical cord blood (UCB) had a success rate of only 26% and is frequently contaminated by CD271 negative osteoclast-like cells. So it is possible that CD271 isolation can enhance the isolation rate of MSCs from UCB. Materials and Methods: 12 samples of 50cc UCB were collected under sterile condition. Cells were isolated on Ficoll layer. Half of the samples underwent CD271 isolation using magnetic activated cell sorting (MACS) technique. The other half used as the control. All the cells were culturedinMesencult media. MSCs isolation was confirmed with respect to morphology, flowcytometry, adipogenic and osteogenic differentiation potentials. Results: CD271 positive portion did not yealed any outgrowth (0/6) despite 3/6 MSCs enrichment in the vontrol group. No osteoclast-like cell was noted in CD271+ portion of cells but 50% contamination noted in control group. Conclusion: Also CD271+ cells can be seen using flowcytometry method in UCB but since not all of these cells are MSCs, the procedures of MACS can eliminate the very rare MSCs in UCB and this will reduce the rate of success.