P-151: Effects of Ultrasound with Two Different Doses on Skin Wound Healing in Rabbits


Ramezani M *, Sadraie SH , Kaka GhR ,

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Objective: Application of some modalities such as ultrasound might be accelerate the process healing of skin wound or surgical incisions. The aim of this study was to examine the effects of ultrasound on the skin wound healing in rabbit. Materiald ans Methods: Twelve adult male Dutch rabbits were divided into two experimental groups (Exp1 and Exp 2) and one control group. After anesthesia a standard skin incision to a length of 3 cm was created on the inner surface of the right leg of rabbits. After three days of surgery the rabbits of Exp1 and Exp2 groups received respectively ultrasound with 1MHz & pulsed 1:1 & 100 and 300 mw/cm2 for 15 min/day. The rabbits of Control group have no received any treatment. The rabbits were killed at week 4 post surgery by inhalation of chlorophorm. For histological examination the samples of skin were removed, fixed and processed. Histological sectioned at 5 microns stained with hematoxylin - eosin (H&E) and massons trichrome. Histomorphometric evaluation of surface of the collagen tissue in the wound area and the number of fibroblast cells were studied in all groups. The data were analyzed by using student t-test. Results: The mean number of fibroblast cells in the Exp1 group was 25.7 ± 2.89 and in the Exp2 group was 27.9 ± 2.56 and in the control group was 27.0 ± 1.73. There are no significant differences between Exp1 and Exp2 with control groups (p<0.05). In addition to, the mean area of collagen tissue in the Exp1 group was 1,100,594 ± 660.26 and in the Exp2 groups was 1, 124, 788 ± 144.43 and in the control group was 1, 406, 989 ± 420.72. There are no significant differences between Exp1 and Exp2 with control groups (p<0.05). Conclusion: These results showed that wound skin was not sensitive to this level of ultrasound application and it had no effect of wound healing of rabbit skin.