Expression of COLLAGEN 1 and ELASTIN Genes in Mitral Valvular Interstitial Cells within Microfiber Reinforced Hydrogel


Maryam Eslami, M.D, Ph.D., 1,2,3,*Gholamreza Javadi, Ph.D, 1,*Nasser Agdami, Ph.D, 4Mohammad Ali Shokrgozar, Ph.D, 5
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Department of Genetics,Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Applied Biotechnology Research Center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Cell Bank Division, Pasteur Institute of Iran (IPI), Tehran, Iran
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Department of Genetics,Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Applied Biotechnology Research Center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Cell Bank Division, Pasteur Institute of Iran (IPI), Tehran, Iran
*Corresponding Address: P.O.Box: 1477893855 Department of Biology Science and Research Branch Islamic Azad University Tehran Iran Emails:maryam.eslami2010@gmail.comgdjavadiroberts@yahoo.com
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Abstract

Objective

The incidence of heart valve disease is increasing worldwide and the number of heart valve replacements is expected to increase in the future. By mimicking the main tissue structures and properties of heart valve, tissue engineering offers new options for the replacements. Applying an appropriate scaffold in fabricating tissue-engineered heart valves (TEHVs) is of importance since it affects the secretion of the main extracellular matrix (ECM) components, collagen 1 and elastin, which are crucial in providing the proper mechanical properties of TEHVs.

Materials and Methods

Using real-time polymerase chain reaction (PCR) in this experi- mental study, the relative expression levels of COLLAGEN 1 and ELASTIN were obtained for three samples of each examined sheep mitral valvular interstitial cells (MVICs)-seeded onto electrospun poly (glycerol sebacate) (PGS)-poly (ε-caprolactone) (PCL) microfibrous, gelatin and hyaluronic acid based hydrogel-only and composite (PGS-PCL/hydrogel) scaffolds. This composite has been shown to create a synthetic three-dimensional (3D) microenvironment with appropriate mechanical and biological properties for MVICs.

Results

Cell viability and metabolic activity were similar among all scaffold types. Our results showed that the level of relative expression of COLLAGEN 1 and ELASTIN genes was higher in the encapsulated composite scaffolds compared to PGS-PCL-only and hydrogel-only scaffolds with the difference being statistically significant (P<0.05).

Conclusion

The encapsulated composite scaffolds are more conducive to ECM secretion over the PGS-PCL-only and hydrogel-only scaffolds. This composite scaffold can serve as a model scaffold for heart valve tissue engineering.