Expression of COLLAGEN 1 and ELASTIN Genes in
Mitral Valvular Interstitial Cells within Microfiber
The incidence of heart valve disease is increasing worldwide and the number of heart valve replacements is expected to increase in the future. By mimicking the main tissue structures and properties of heart valve, tissue engineering offers new options for the replacements. Applying an appropriate scaffold in fabricating tissue-engineered heart valves (TEHVs) is of importance since it affects the secretion of the main extracellular matrix (ECM) components, collagen 1 and elastin, which are crucial in providing the proper mechanical properties of TEHVs.
Materials and Methods
Using real-time polymerase chain reaction (PCR) in this experi-
mental study, the relative expression levels of
Cell viability and metabolic activity were similar among all scaffold types. Our
results showed that the level of relative expression of
The encapsulated composite scaffolds are more conducive to ECM secretion over the PGS-PCL-only and hydrogel-only scaffolds. This composite scaffold can serve as a model scaffold for heart valve tissue engineering.