Effect of Sperm DNA Damage on Fertilization Rate Post ICSI

Razavi S *, Nasr Esfahani MH , Babazadeh Z ,


Objective: The first step for evaluation of male infertility is semen analysis but many studies suggest that other parameters such as sperm DNA integrity have more effects on ICSI outcomes. The aim of this study was to evaluate the correlation sperm DNA integrity using SCD and Comet assay with fertilization rate post ICSI. Materials and Methods: DNA damage in sperm of 41 men undergoing ICSI treatment was measured by comet assay and SCD test. Around 16–18 h post ICSI fertilization was assessed by presence of pronuclei and the percentage of fertilization was calculated by the ratio of fertilized oocytes to the total number of survived injected metaphase II (MII) oocytes multiplied by 100 . Coefficients of correlation were calculated using SPSS 11.5 software. Results: No significant correlation was obtained between fertilization rate and sperm DNA damage measured by SCD (p=0.091) and Comet assay (p=0.612) in ICSI patients . Conclusion: Our results show that the sperm DNA damage doesn't directly effect on fertilization rate since damaged DNA carried into the zygote by the fertilizing spermatozoon will be repaired by the oocyte. However it can be effects on subsequent embryo development. It could be devised to identify and select spermatozoa with intact DNA or to remove spermatozoa with damaged DNA so as to improve ICSI outcomes.