The Effect of Hydrostatic Pressure on In Vitro Maturation of Oocytes Derived from the Stimulated and Unstimulated Ovary (Pages: 0-0)


Momeni M *, Azadbakht M , Amini A ,

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Objective: Oocyte maturation is a process that during which the oocyte attains the competence to be fertilized. For clinical application most oocytes are obtained from ovarian follicles of women who have been treated with hormones. Side effects of superovulation are ovarian hyperstimulation syndrome or polycystic ovary syndrome. Hence there is an increasing interest in retrieving oocytes without gonadotropins and then in vitro maturation. Hydrostatic pressure as a physical force is effective on reproductive system. Obviously there is an increase in intrafollicular pressure 15-20 mmHg in the ovulating follicle during the late stage of the ovulatory process. In this study we examined the effect of hydrostatic pressure on in vitro maturation of oocytes derived from the stimulated and unstimulated ovary. Materials and Methods: In this study we had two experimental groups. In experiment I female NMRI mice 6-8-week-old received 10 IU/ml PMSG hormone and in experiment II did not. Preovulatory follicles were isolated from mice ovaries each follicle cultured individually in micro<font>drop</font>s of MEM-α supplemented with 5% fetal bovine serum 100-mIU/ml rFSH(Gonal-f) 10ng/ml recombinant Epidermal Growth Factor 7.5 mIU/ml HCG under mineral oil for maturation. At the start of in vitro maturation follicles from two experiments divided into two treatments. In treatment I follicles were transferred to pressure chamber and exposed to 20 mmHg hydrostatic pressures for 30 min and in treatment II follicles were transferred to pressure chamber without hydrostatic pressure exposure. Then follicles were cultured for maturation of oocyte. Results: After 24 h in experiment I percent of MII oocyte was increased in follicles with hydrostatic pressure exposure (15.38%) compared to follicles without hydrostatic pressure exposure (10%) (P