Reduction of Induced Transgenerational Genomic Instability in Gametes Using Vitamins E and C Observed as Chromosomal Aneuploidy and Micronuclei in Preimplantation Embryos


Mozdarani H *,

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Objective: Mutational events may be an indirect effect on genome stability which is transmitted through the germ line of chemically or physically exposed parents to their offspring. The consequences of germ cell mutations in subsequent generations include genetically determined phenotypic alterations without signs of illness or reduction in fertility or embryonic or prenatal death more or less severe congenital malformations or genetic diseases with various degrees of health impairment. In this study the effect of induction of DNA damage during spermatogenesis cycle and preovulatory stage oocyte on the frequency of chromosomal abnormalities and micronuclei formation in preimplantation embryos generated by damaged sperm or oocyte after exposure to gamma rays in the presence or absence of vitamins E and C is investigated. Materials and Methods: DNA damage was induced in male NMRI mice using gamma-rays and then mated with non irradiated super-ovulated female mice in 6 successive weeks after irradiation in a weekly interval. In experiments involving irradiation of both male and female mice irradiated male mice for 6 weeks post-irradiation were mated with female mice irradiated after induction of super-ovulation. To study the effects of vitamins E and C on the radiation induced DNA damage and consequently in the chromosomal abnormalities generated in preimplantation embryos vitamin E at a concentration of 200 mg/kg and vitamin E at 100 mg/kg was administered to mice interaperitoneally 1 hour before exposing to radiation. Standard methods were used to prepare slides from pre-embryos for chromosome and micronuclei study. Results: The rate of both aneuploidy and MN observed in embryos generated from irradiated male compared to control group dramatically increased (p