Objective: Testicular torsion is the twisting of the spermatic cord which cuts off the blood supply to the testicle. The main pathophysiology of testicular torsion is ischemia-reperfusion injury caused by the twisted spermatic cord and its release. Reperfusion component typically involves the generation of the reactive oxygen species with the return of blood flow after the ischemia. The aim of the present study was to investigate the effects of dopamine a vasodilator and vitamin C an antioxidant on ischemia reperfusion injury due to testicular torsion-detorsion (T/D). Materials and Methods: Thirty adult male NMRI rats were divided randomly into six groups each containing 5 rats. Testicular ischemia was achieved by twisting the left testis 720° clockwise for 4 hour and then reperfusion by detorsion. Group 1 was for the basal values. Group 2 was designed as sham-operated without any T/D. Group 3 had only T/D. Group 4 received vitamin C (100 mg/kg i.p) just after detorsion. Group 5 received dopamine (0.01 mg/kg i.p) after 4 hours T/D and group 6 received combination of vitamin C and dopamine. The animals in each group underwent orchiectomy 48 hours after detorsion and the testicular tissues were fixed in 10% formaldehyde for histopathological examinations. Results: In groups 1 and 2 microscopic features were normal. In group 3 all of the seminiferous tubules were damaged. In some tubules only the basement membrane was seen. Pyknosis of spermatocytes nuclei was prominent. Germ cells and sertoli cells were decreased and underwent degeneration and necrosis. Interestingly in group 4 only a few degenerative changes (6.8%) were observed in tubules. In group 5 approximately 90% of tubules were underwent fully or partial degeneration and in group 6 only few normal tubules were found (2.2%). Conclusion: Vitamin C administration exerts a beneficial effect on testicular ischemia-reperfusion injury but not dopamine or even dopamine and vitamin C combination.