Developmental Effects of Lead Acetate on Seminiferous Tubules in Wistar Rats


Dorostghoal M *, Moazedi , Sorooshnia F ,

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Objective: In recent years concerns have been raised about the decrease of male infertility from exposure to environmental pollution such as heavy metals. So developmental effects of lead acetate were studied on seminiferous tubules structure during postnatal development in male Wistar rats. Materials and Methods: The male pups randomly divided in control and experiment groups. The mothers of experiment groups received 100 200 and 300mg/L/day lead acetate via drinking water during lactation. The testes of pups removed their weights were recorded and fixed in Bouin’s solution. Then the volumes of testes were measured by Cavalieri method. For microscopic studies following tissue processing 5µm sections were stained with haematoxylin-eosin. Results: Macroscopic results showed that the means of testis weight of experiment groups increase during early and decrease at the late of postnatal development in comparison with control group. Microscopic results indicated that decreasing of germinal epithelium height and presence of vacuoles in germinal epithelium comparison with control group. Also the means of testis volume relative volumes of seminiferous tubules and germinal epithelium significantly decreased in experiment groups during all stage of postnatal development (p