Stereological Effects of Di (2-Ethylhexyl) Phthalate on Postnatal Development of Testis in Wistar Rat


M. Dorostghoal *, Moazedi , F. Sorooshnia ,

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Objective: Phthalate esters extensively used as plasticizers in the plastic industry have been shown to cause reproductive toxicity in both developing and adult animals. So present study was aimed to determine quantitative effects of di-(2-ethylhexyl)-phthalate (DEHP) on postnatal development of rat testis. Materials and Methods: For this purpose pregnant females rat were orally exposed to various doses (0 100 and 500 mg/kg per day) of DEHP during gestation and lactation. Testes of offspring were removed at 7 14 28 60 and 90 days after birth their weight recorded and fixed in bouin’s solution. Then the volumes of testes were measured by Cavalieri method. For microscopic studies following tissue processing 5 μm sections were stained with haematoxylin-eosin. Results: The results were showed that the means of testis weight decreased significantly (p