The Effect of Various Doses of Nicotine on Spermatogenesis Maturation and Sperm Parameters in Adult Mice (Pages: 0-0)


Fallah M *, Ghasemi F.M , Ranjbar S , Jalalsafari M ,

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Objective: Cigarette smoking is associated with impairment of testicular function. Nicotine is a major component of cigarette smoke. In the present study we aimed to demonstrate the effect of various doses of nicotine on sperm parameters and quality of spermatogenesis in adult mice. Materials and Methods: Male adult NMRI mice were divided into four groups. 1) control group were received normal salin. The animals in experimental groups of 2 3 and 4 were received nicotine at the doses of 0.1 0.2 and 0.4 mg/100 g. BW respectively. Both nicotine and vehicle were administered intraperitoneally for 21 days. Evaluations were made by determining Johnson's score epididymal sperm content motility and morphology leydig cells volume and serum concentration of testosterone. Statistical analysis was performed by ANOVA test. Results: Nicotine caused a significant reduction in quality of spermatogenesis sperm content leydig cell volume and serum concentration of testosterone in groups of 3 and 4( p