Testosterone Enanthate Suppresses Apoptosis in Male Germ Cells Induced by Busulfan (Pages: 0-0)


Ghasemi F.M1 *, Faghani M , Nasiri E , Bahadori M.H , Soltani B ,

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Objective: This study was performed to investigate the suppressive effect of testosterone enantate on male germ cells apoptosis induced by busulfan in rats. Materials and Methods: Sprague- Dawley rats were divided into 4 groups: 1. a control group given cotton seed oil 2. a group given two doses of busulfan 10 mg/kg intraperitoneally 21 days apart 3. a group given two doses of testosterone enantate 8mg/kg subcutaneously 21 days apart 4. a group given both testosterone anantate and busulfan. Evaluations were made by determining johnsen’s score and apoptosis were assayed by terminal- deoxynucleotidyl- transferase-mediated dutp nick end labeling (TUNEL). Results: Recovery status and johnsen’s score in group 4(busulfan + testosterone) were significantly higher than group 2(busulfan) 7.4 ± 0.20 VS 4.1 ± 0.22 TUNEL positive cells were significantly more numerous in busulfan treated group than control. While testosterone significantly attenuated morphological changes and germ cell apoptosis in testis damaged by busulfan. Conclusion: These results suggest testosterone enantate have chemoprotective effect on male germ cells against busulfan-induced toxicity partly by decreasing of apoptosis.