Effect of Growth Hormone on Testicular Dysfunction Induced by Methotrexate (MTX) in Rats (Pages: 0-0)

Serati Nouri H *, Azarmi Y , Movahedin M ,


Objective: Methotrexate (MTX) is a chemotherapeutic agent causing defective oogenesis and spermatogenesis. This study was planned to see the role of human growth hormone (GH) on testis recovery after treatment with methotrexate. Materials and Methods: The forty male Wistar rat were selected and randomly divided into four groups (n=10); control (vehicle) GH group (0.3mg/kg GH for 28 day IP) MTX Group (MTX 1mg/kg /week for four week IP) and GH/MTX group (0.3mg/kg GH for 28 day plus 1mg/kg /week MTX for four week IP)). During day 14 and 28 five rats from each group were sacrificed and the testes of rats in all groups were removed and sperm was collected from epididymis and then prepared for analysis. Results: MTX causes significant increase in interstitial tissue and capsular thickness and decrease of testicular and body weight (p