Effects of Alcohol and Nicotine Co-Administration on Rat Sperm Parameters

Saeid Azizollahi *, Arash Saveazad , Seyed Noureddin Nematollahi-Mahani , Masoud Ezzatabadipour , Zohreh Mirkahnooj ,


Objective: Toxic effect of alcohol and nicotine on the other organs of body has been proven to some extent. Effects of oral alcohol and subcutaneous nicotine co-administration on rat sperm parameters are the aim of this study. Materials and Methods: Thirty healthy Spauge-Dawley male rats (average weight 160-210 g) were randomly divided to 3 groups (n=10). The animals in the first group received the same volume of vehicle for 50 days and served as control. The second group received daily subcutaneous injection of 0.02 mg nicotine and daily oral gavage with 2 ml 20% Ethanol simultaneously. The third group was intact group received no intervention and served to evaluate probable age changes in the reproductive system and to consider any stress induced by the treatments. For Sperm analysis Cauda epididymis and vas deference were placed in 5 ml α-MEM supplemented with 2% BSA and cut into several fragments to allow the spermatozoa come out from reproductive ducts. Samples were incubated for 15 min. at 37○C and the following experiments were carried out on sperm suspensions in various groups. Sperm quality was determined by three parameters; concentration motility and viability. The viability was assessed by three methods; Eosin exclusion dye test Hypo-osmotic swelling test and Propidium iodide exclusion test. Results: Sperm concentration was comparable between intact and control groups while a reduction in sperm concentration was observed in ethanol/nicotine group compared to both intact and control groups (p