Effect of Hydrostatic Pressure Exposure to Sperm on In Vitro Fertilization


Bahrami Kamangar P *, Azadbakht M , Amini A , Amiri I ,

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Objective: In vitro fertilization is an effective treatment to assist infertile couples. Sometimes infertility is because of male factors. Different factors have effect on sperm and causes infertility. Sperm from formation in male reproductive system until conception in female reproductive system is exposed to different factors. Increase or decrease in normal range of these factors causes damage on sperm and may causes infertility. Hydrostatic pressure is a crucial component of cell environment and fundamental physical quantity; also it is the main factor of integrity and function of cells. Pressure variation disorders beyond physiological limits may lead to infertility and subfertility. Any prejudiced about fertility or subfertility is based on results of sperm vital parameters including viability motility and morphology. In this study we examined the effect of hydrostatic pressure exposure to sperm on in vitro fertilization. Materials and Methods: In this study we used female NMRI mice with 6-8 weeks old and male mice with 8-12 weeks old. The females were superovulated by 10 IU intraperitoneal injection of PMSG followed by 10 IU HCG 51 h later. The females were sacrificed by cervical dislocation 16 h after the HCG injection and their oviducts removed and then MII oocytes were transferred into a plastic culture dishes containing HTF medium. Sperms separated from the epididymis and maintained in the Ham's F-10 culture medium supplemented with 10% FBS. Sperm suspension in the experiment group were placed within pressure chamber and pressurized into 100 mmHg for 4 h. Sperm vital parameters including morphology viability and motility were evaluated and compared with control group. Then in vitro fertilization rate were studied. Results: Our results showed that hydrostatic pressure reduced sperm viability (p