Effects of Repeated Subsquent Gonadotropin Adminatration on Endometrium Diameter and Ovarian Homoral Response (Pages: 0-0)


Heidari F *, Sadrkhanlou R , Rastegarnia A , Ahmadi A , Ghafari S , Raberi A , Kargar K ,

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Objective: Gonadotropin has been widely used word wide for many years to induce ovulation .three main exogenous gonadotropins are used for follicular development and ovulation induction –follicle stimulating hormone(FSH)luteinizing hormone(LH)and human chronic gonadotropin(hCG).Currently these gonadotropins or their recombinant are present in the urine(with the exception of (LH).Other authors have reviewed the physiology of the ovulatory cycle and the role of the gonadotropin in ovulation induction in patient with an-ovulatory disorders and in multi-follicular development for assisted reproductive technologies. The aim of this experiment is to find the optimal subsequent time for repeated gonadotropin administration for the maximum response for ovarian follicular development Materials and Methods: Twenty five female mature Syrian mice of 6 weeks old were used to determine the optimal subsequent period for repeated gonadotropin administration for the raising of ovarian follicular estrogen level and measurement of the endometrioum and epithelial layer thickness and gland dispersion.The animals were housed at animal house with 12hours light 12 hours dark periods daily at temperature 22 with free access to food and water adlibidum.The animals were divided in to 5 groups all groups received 10 (IU)PMSG(folligon )IP and following 48 hours of hormone treatment the first group of animal(check) was sacrificed and the blood was collected for estrogen assay and uterine horns were dissected and processed for tissue embedding and H&E staining to evaluate with hormonal response.The other four groups were injected 10 IU PMSG at 246and 8 week later subsequently and their plasma estrogen level were measured by electrochemilumiescenc( ECL)assay technique Results: The result of this experiment has shown that beginning from the third group the level of estrogen hormones in blood plasma and endometrium and epithelial layer thickness together with gland dispersion that was measured by light microscope was raised at its maximum amount and remained constant at the two other groups which were significantly higher than the control groups. Conclusion: This experiment has shown that the optimal time following first gonadotropin treatment cancellation for the next gonadotropin administrated is four weeks after first hormone treatment with regarding to the estrous cycle period in the mice which is about 4-5 days. The optimal time for repeated subsequent gonadotropin administration for ovarian follicular development is about four weeks from former PMSG injection