Objective: Concentrations of certain substances in follicular fluid (FF) may related to fertilization outcome. The study aim was to identify FF markers with which to predict embryo implantation potential. Materials and Methods: Concentrations of selected hormones cytokines and growth factors in FF samples obtained during assisted reproduction treatment Intracytoplasmic Sperm Injection (ICSI) method were related with treatment outcomes. Results: Mean concentrations of 17 beta-estradiol (E2) prolactin (PRL) and insulin-like growth factor (IGF)-I were significantly higher in treatment attempts leading to a clinical pregnancy as compared with those in which no pregnancy was established. LH growth hormone (GH) progesterone and interleukin-1 (IL-1) concentrations are similar in successful and unsuccessful attempts. Conclusion: Fertilization outcomes were related to FF levels of PRL E2 and IGF-I. But LH GH progesterone and IL-1 are not markers of success.