Adverse Effects of Hydrostatic Pressure on Sperm Vital Parameters (Pages: 0-0)


Karimi N *, Kamangar P , Azadbakht M , Amini A , Amiri I ,

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Objective: Studies indicated that 50 percent of infertility in couples is related to males. Prejudiced about fertility or subfertility based on results of sperm vital parameters including viability morphology and motility. In males different parameters are identified as cause of infertility and subfertility such as environmental causes including: physical-mechanical and sperms own causes including: reduce of sperm number abnormality in morphology and motility and changes in the sperm chromatin condensation. Hydrostatic pressure is a crucial component of cell environment and fundamental physical quantity; also it is the main factor of both integrity and function of cells. Sperms exposed to different range of hydrostatic pressure within male reproductive system and after fertilization and enter to the female reproductive system. Pressure variation disorders beyond physiological limits may lead to infertility and subfertility .This study examined the effects of pathological range of hydrostatic pressure on sperm in aspect of survival morphology and motility. The objective of this study was to develop and understanding of the link between abnormalities in sperm that induced by hydrostatic pressure in relation to reduce of male fertility Materials and Methods: Sexually mature NMRI male mice ages of 8-12 weeks-old were as sperm donors. Mouse sperms separated from the cauda epididymis and maintained in Ham's F-10 culture medium supplemented with 10% FBS and divided into two groups as control and experiment. Sperm suspension in the experiment group were placed within pressure chamber and pressurized into 100 mmHg for 2 and 4 h sperm vital parameters were evaluated and compared with control group. Results: Our results showed that hydrostatic pressure changed sperm viability gradually and reduction happened significantly after 4 h (p