Effect of Ascorbic Acid on In Vitro Fertilization of Mouse Oocyte and Embryo Development to the Bastocyst Stage

Moayedi A *, Niyazi E , Bahadori M.H , Heidarzadeh A , Ahmadi M , Ahmadi M ,


Objective: Infertility is a main problem of approximately 15 % of couples trying to conceive in a period of their lives. In the past decades in vitro fertilization and its related techniques have been an appropriate method to cure infertile patients. Studies have shown that in present in vitro conditions rate and speed of embryo development cell number their synthetic activity and their biological competence is less than those which developed in in vivo environment. One of its major causes is generation of reactive oxygen species (ROS) in in vitro cultures that results in degrees of infertility. Ascorbic acid is a kind of hy<font>drop</font>hilic antioxidants in the follicular fluid that can scavenge oxygen metabolic like hydrogen peroxide super oxide anions and hydroxyl radicals from the environment. So regarding above this study conducted to evaluate different concentrations of ascorbic acid on the improvement of embryo development till the blastocyst stage. Materials and Methods: Two pronucleous (2 PN) zygotes were obtained from female NMRI mice after administration of i.p. injection of 5 IU Pregnant Mares Serum Gonadotropin (PMSG) and subsequent human Chorionic Gonadotropin (hCG) injection. Groups of 2 PN zygotes were randomly placed in T6 + BSA 16 mg/ml medium <font>drop</font> without or with ascorbic acid(100 400) and halved doses(50 200) supplemented at 24 and 48 hours they were cultured to the hatched blastocyst stage and the number of embryo in different stage was recorded under an invert microscope and compared. Results: In this study Addition of Ascorbic acid to embryo culture media promoted the development from 2 PN stage embryos to morula blastocyst and hatched blastocyst. The addition of ascorbic acid in 50ng/ml supplemented at 24 and 48 into the culture medium increased the percentage of 2 PN mouse embryos that developed into blastocysts and hatched blastocysts significantly whereas in the presence of 100 200 and 400 was not significantly higher. Conclusion: Ascorbic acid plays an important role in the development of preimplantation embryo. It can promote embryo development