Objective: Artemisia absinthium is a species of wormwood native to temperate regions of Eurasia and Middle East.To evaluate possible fertility activity of Artemisia absinthium flowers extract in male rats. Materials and Methods: 24 Male rats(70-90 days old) were randomely divided into 3 groups. Animals in group A(Control group) were administered with the distill water only. Animals in group B and C were administered with oil extract of A. absinthium flowers at the doses of 200 and 400 mg/kg/day for 50 days. At the end of 50 treatment period Body weight Epididymis weight GSI(gonadosomatic index) sperm motility sperm viability ESR (epididymal sperm reserves) DSP(daily sperm production) and testosterone concentration were assessed. Fertility percentage was evaluated with mating test. Results: There was a significant decrease in the GSI sperm motility sperm viability ESR DSP testosterone concentration and Fertility percentage especially in higher dose. Conclusion: Our data concluded that the extract of A. absinthium flowers at both doses(200 and 400 mg/kg) had a negative impact on the fertility parameters.