Relevance of LIF and EGF on Mouse Preimplantation Embryo Development (Pages: 0-0)

Maryam Parvini *, Iraj Amiri , Khadijeh Heidarbeigi ,


Objective: Recent evidence suggests that Leukemia inhibitory factor (LIF)a member of interleukin-6 family has biological actions on preimplantation embryo development.Also it is established that Epidermal growth factor(EGF) is a strong mitosis-promoting agent that improve the preimplantation embryo development by increasing the cell metabolism and proliferation. The purpose of the present study was to investigate the effects of these factors in combination together on preimplantation embryo development. Materials and Methods: Six to eight weeks old NMRI mice were super ovulated by injection of 10IU PMSG and 10 IU hCG 46-48h later. The mated mice were killed 46 hours after hCG. injection oviducts were flushed and two-cell embryos collected and divided randomly to four groups as following: Control) treatment 1 (LIF) treatment 2 (EGF) treatment 3 (LIF+EGF).In each group the embryos were cultured in an incubator at 37°C with 5% CO2 and 90% humidity for 72h.The state of embryo development was evaluated in 24364860 and 72 hours following culture. At the end of culture cell apoptosis was studied by the terminal deoxynucleotidyl transferas-mediated dUTP nick end-labeling(TUNEL) technique. Results: Significant difference was detected in the rate blastocyst formation after 36 hours in the LIF and LIF+EGF groups (p