The Effects of Electromagnetic Field on Mouse Blastocyst Apoptosis


Rajaei F *, Borhani N , Shams A.R ,

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Objective: The growing development of electronic industries and the increasing use of electrical appliances have led to higher rise in chronic exposure of people to extremely-low-frequency electromagnetic field (ELF-EMF). The aim of the present study was to investigate the possible effect of electromagnetic field exposure on DNA fragmentation in mouse blastocysts Materials and Methods: Eighty female NMRI mice were randomly divided into 2 groups of 40 animals: Control group was left unexposed. EMF group was exposed to 50 Hz & 0.5 mT EMF for 4 hours per day 6 days a week and a total duration of 2 weeks. On the 8th day of exposure the female mice in both groups were superovulated and mated overnight. In the next morning the female mice with a vaginal plug were identified as pregnant; at the time of implantation the pregnant mice were killed and blastocysts obtained by flushing the uterus horns. The mean number of pregnant mice blastocysts after flushing and the DNA fragmentation index following TUNEL staining in both groups were compared using statistical methods (SPSS t test p