Secretion of VEGF Following three Dimensional Culture of Human Endometrial Tissue: an In Vitro Endometriosis Model (Pages: 0-0)


Ai J *, Esfandiari N , Casper R.F ,

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Objective: Endometriosis is the presence of endometrial glandular and stromal cells outside of the uterine cavity. This disease is found in about 10% of women of the reproductive age and in up to 50% of women with infertility. Surgery continues as the first-line treatment to eradicate endometriotic lesions but recurrence of the condition occurs in up to 47% of women.VEGF is an effective factor in establishment of endometriosis. The aim of the present study is to determine VEGF levels in human culture of endometrial fragment Materials and Methods: Endometrial biopsy in Premenopausal patient’s women referred to T.C.A.R.T (Toronto Centre for Advanced Reproductive Technology) for infertility treatment such as uterine myomas or ovarian cyst. Endometrial samples were collected from a total of ten normal ovulating women on cycle days 19-24. The biopsies were obtained from the fundal region of the uterine cavity. Ten tissue fragments were cultured by three dimensional methods for each patient. Supernatant fluid sample was collected from endometrial samples which were cultured in a three-dimensional fibrin matrix. Results: Level of VEGF in Supernatant fluid of endometrial samples was placed in a three-dimensional fibrin matrix culture system were determined. These data showed Cell proliferation was observed in 91% of the wells. Angiogenesis was observed in 51 wells that showed cell proliferation (56%). The level of PRL in the supernatant fluid of wells that showed angiogenesis were increased (P