Observation of Satellite Association in Couples with History of Habitual Abortion (Pages: 0-0)


Manoochehry F *, Razazian F , Mortezapour F , Rahnama M , Nasiri F , Mahjoubi F ,

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Objective: Here we report a very rare and interesting case with a constitutional break or gap at site (16q22.3). A couple refereed to our laboratory for chromosome study due to the history of miscarriage and still birth. The woman had a normal karyotype. The man had 4 cell lines. One cell line had a breakage at chromosome 16q22.3, one cell line with the deletion at 16 q22.3, one cell line with two normal chromosome 16 and a marker chromosome (very likely originated from 16q22.3). The last cell line had apparently normal male karyotype. To our best knowledge there are only two other case with the same break or gap at 16q22 resulting to abortions in both cases and a full term delivery with a Down syndrome child in one case. The phenotype of our case was normal because the cells with deletion and with marker of 16q22.3-qter outbalance each other. It is very likely that the deletion and marker cell lines emerged in culture and would die out in the next cycle. Thus, the major problem is that of spontaneous abortions. The couples advice to have genetic counseling and prenatal diagnosis for the future pregnancies. Materials and Methods: Lymphocyte cultures from the patients were set up in RPMI1640 supplemented with 20% FBS.high resolution chromosome banding was performed in all subjec Results: Thus, the major problem is that of spontaneous abortions. The couples advice to have genetic counseling and prenatal diagnosis for the future pregnancies. Conclusion: The phenotype of our case was normal because the cells with deletion and with marker of 16q22.3-qter outbalance each other. It is very likely that the deletion and marker cell lines emerged in culture and would die out in the next cycle.