Objectives: Considering that certain cytokines may change during pre-eclampsia (PE), because of functional polymorphisms in their genes, our purpose was to determine the association between tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) gene polymorphisms and development of PE. Materials and Methods: The genetic polymorphisms of TNF-alpha and IL-10 was studied by polymerase chain reaction sequence specific primers (PCR-SSP) in the DNA of PBC from 160 patients with PE and 100 healthy pregnant women. SPSS software, version 11.5, was used for statistical analysis. Association analysis was used in order to identify any relationship between the alleles and the occurrence of PE versus normal pregnancy in patients. Results: We found a significant difference between TNF-alpha A allele (-308) and G allele (-238) in PE patients compared with those of the control groups. A significantly higher C⁄C genotype frequency of IL-10 (-592) and (-819) was observed in the PE patients than in the control groups. In addition, the frequencies of three common IL-10 haplotypes (GCC, ACC, and ATA) did not show any significant difference between the study groups. Conclusion: These findings would support the concept of contribution of TNF-alpha and IL-10 gene polymorphisms in the pathogenesis of PE in our population.