Efficient Replacing of Fetal Bovine Serum with Platelet Lysate during Propagation and Differentiation of Human Bone Marrow Derived Mesenchymal Stem Cells to Functional Hepatocytes-like Cells (Pages: 0-0)


Allameh A *, Kazemnezhad S , Soleimani M , Gharehbaghian A , Amirizadeh N ,

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Objective: The studies in stem cell biology have shown that mesenchymal stem cells (MSCs) can differentiate into hepatocytes. Traditionally, fetal bovine serum (FBS) has been used for expansion and differentiation of MSCs to different lineages. However, certain risks associated with the use of FBS have stimulated a search for alternatives. The aim of this study was to find out substitution effect of FBS with human platelet releasate (HPR) as a major growth factor source during expansion and differentiation of human bone marrow derived MSCs into hepatocytes. Materials and Methods: For this purpose, parallel experiments were carried out using either the conventional culture media (containing FBS) or HPR- supplemented media. Results: MTT assay showed that HPR was more efficient than FBS in supporting hBMSC outgrowth. The proliferation rate of MSC in presence of HPR (derived from 109 platelets/ml) was about 3-fold greater than that of FBS. Despite these differences in MTT value, hBMSCs driven HPR and hBMSCs driven FBS did not differ in terms of gross morphology, immunophenotype and osteogenic differentiation potential. The evidences presented in this paper show that the hepatic differentiation of hBMSCs, was successfully completed in the media enriched with HPR. Immunoreactivity of cells with monoclonal antibodies against hepatocyte markers such as albumin and alpha-fetoprotein (AFP) as judged by immunofluorescent staining was even more positive in hepatocytes differentiated in presence of HPR as compared to that of FBS (p