Amniotic Fluid Versus Bone Marrow Derived Mesenchymal Stem Cells: Proliferation, Bone Differentiation and Senescence (Pages: 0-0)

Jahangir Sh *, Baghaban Eslaminejad M.R , Aghdami N ,


Objective: Mesenchymal stem cvells (MSCs) have been known as an appropriate candidate for cell replacement therapy of tissue defects including those associated with skeletal system. These cells so far have been isolated from several tissue sources as bone marrow(BM) and amniotic fluid(AF), but little is known about the differences existed between the cells from these sources. This subject was considered in the present investigation. Materials and Methods: Amniotic fluid and bone marrow cells were collected from NMRI mice and culture expanded through several passages. To ensure the MSCs nature, isolated cells were differentiated among bone, cartilage and adipose cell lineages. Moreover, the cells from two sources were compared to each other in terms of the proliferation rate, the potential of bone differentiation and the senescence in culture by calculating population doubling time (PDT), quantification of culture mineralization and determining the culture beta-galactosidase positive cells respectively. Results: Culture-expanded cells from either source appeared to be able to generate bone, cartilage and adipose cells upon providing with appropriate inductive microenvironment hence being MSCs in nature. The PDT value for AF MSCs was significantly less than that for BM MSCs (92.6 ± 13.9 versus168 ± 40 hours, p