Proliferation and Aging of Rat Mesenchymal Stem Cells from Epicardial Adipose Tissue in Comparison to those from Bone Marrow Tissue (Pages: 0-0)

Mardpour S *, Baghaban Eslaminejad M.R , Ebrahimi M ,


Objective: Mesenchymal stem cells (MSCs) have first been isolated and described from bone marrow tissue. Subsequent studies have indicated the existence of these cells in many other tissues. Little is known regarding the differences between MSCs from different sources. In this study MSCs from adipose tissue (AT) were compared to those from bone marrow (BM) samples. Materials and Methods: For this purpose AT was obtained from rat epicardial tissue and subjected to enzymatic digestion. Released cells were then plated and culture expanded through several passages. In parallel, expansion cultures were established for rat BM cells for several subcultures. To ensure the MSCs nature, isolated cells were differentiated among bone, cartilage and adipose cell lineages. To compare the proliferation rate of the cells from two sources, population doubling time (PDT) and colonogenic activity were determined for either culture. Beta galactosidase assay was used to compare the MSCs from two sources in terms of their culture senescence. Results: Culture-expanded cells from either source were able to produce bone, cartilage and adipose cell lineages (evidenced by RT-PCR analysis for related specific genes) confirming that they were the MSCs described elsewhere. According to our results AT cells appeared to have a short PDT value than their BM counterparts (45.84 ± 16 versus 318.24 ± 18.24, p