Gene Expression of Catalase during Neural Differentiation of P19 Cells (Pages: 0-0)


Mojbafan M *, Ghaedi K , Razavi S , Tanhaei S , Karamali F , Rabiee F , Nasr Esfahani M.H ,

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Objective: The P19 murine embryonal carcinoma (EC) cell line is a valuable in vitro model cell that can be differentiated into neurons by cellular aggregation in presence of the differentiating agent retinoic acid (RA). Materials and Methods: Total RNA from P19 cells were extracted and cDNA was synthesized. Using specific primers, respective cDNAs were amplified and were analyzed by semi-quantitative RT-PCR Results: In this project, a peroxisomal gene such as catalase has been selected and the profile of its expression has been investigated in P19 cells. Expression of peroxisomal gene like Catalase, as peroxisomal matrix protein in comparison with pluripotency markers such as Oct4 and Nanog, neural markers such as Pax6, Ngn-1, Map2 and a house keeping gene such as β-tubulin have been investigated by RT-PCR Conclusion: Data indicated that during neural differentiation, expression of pluripotency markers have been down regulated while, expression of neural markers was significantly increased. However incase of Catalase gene expression, there was an increase in Catalase gene expression upon Retinoic acid treatment, during neural differentiation, which was observed at the final steps of neurogenesis.