In Vitro Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells into Cardiomyocytes (Pages: 0-0)

Motamedy R *, Azadbakht M , Fathi F , Amini A , Raoufi A , Amini S ,


Objective: The inability of adult cardiomyocyte to divide to a significant extent an regenerate the myocardium after injury leads to permanent deficits in the number of functional cells, which can contribute to development and progression of heart failure. Transplantation of stem cells into the injured myocardium is a novel and promising approached in the treatment of cardiac disease and the restoration of myocardial function. In the present study we investigated the potential of human mesenchymal stem cells from adult bone marrow to differentiate into cardiomyocytes. Materials and Methods: . Human Bone Marrow Mesenchymal Stem Cells (hBMSCs) cultured in enriched medium. hBMSC were treated with 10-6M oxytocin for one month. Morphologic characteristics were analyzed by phase contrast microscope. Expression of hα3-actinin and hβMHC (myosin heavy chain beta) and OTR (Oxytocin Receptor) was detected by RT-PCR. Protein expression of α-actinin and Troponin¬I-C was analyzed through immunostaining. hBMSCs were spindle-shaped with irregular processes. Results: Cell treated with oxytocin were connect with adjoining cells forming myotube like structures. Immonostaining of the differentiated cells for α-actinin and Troponin¬I-C were positive. Conclusion: Based on these observations, we conclude that hBMSCs retain cardiomyogenic potential suitable for cell therapy against intractable heart diseases.