Effects of Dexamethasone (Dex) on Apoptosis in the Mouse Testicular Germ Cells


Orazizadeh M *, Hashemitabar M , Khorsandi L.S ,

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Objective: The aim of the present study was to find out whether Dexamethasone (Dex), a widely used GC, would influence the apoptosis and expression of Bax, an important proapoptotic protein, in the mouse testicular germ cells. Materials and Methods: Experimental groups of 8 male NMRI mice received one of the following treatments daily for 7 days: 4, 7 and 10 mg/kg Dex. Control groups were treated with equivalent volumes of saline. Experimental and control animals were sacrificed 24 h after the last injection. Immunohistochemical procedure was applied to evaluation of Bax expression and statistically evaluated. The deoxyuridine nick-end labeling (TUNEL) was carried out to assessment of the apoptotic germ cells. Results: Apoptotic index was significantly increased in 7 and 10 mg/kg Dex treated mice (p