Generation of Oligodendrocyte Precursors from Human Induced Pluripotent Stem Cells (Pages: 0-0)


Pouya A.R *, Rezaei Larijani M , Taei A , Seifinejad A , Totonchi M , Aghdami N , Baharvand H ,

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Objective: Induced pluripotent stem (iPS) cells have opened a new area for biological and clinical researches. The characteristics of iPS cells are remarkably similar to embryonic stem (ES) cells. However, detailed differentiation properties and the directional differentiation system of iPS cells have not been demonstrated into oligodendrocyte precursors. This study aimed to differentiate human iPS cells into oligodendrocyte precursors.into oligodendrocyte precursors. Materials and Methods: The protocol consisted of an induction of neural-lineage cells by exposing cultures to retinoic acid simultaneous with the preferential selection of oligodendroglial-lineage cells by media components and epidermal growth factor, and the differentiation factor triiodothyroidin hormone (T3). Results: Morphological features and molecular analysis including immunocytostaining consist of Olig2, A2B5, PDGFα-R, NG2, O4 and GalC plus other neural type cell’s markers and RealTime-PCR for Oligodendrocyte markers showed that human iPS and hES cells generate oligodendrocyte precursors. Conclusion: This study showed that oligodendrocyte precursors and their derivatives can be generated from human iPS cells. The ability to generate human oligodendroglia from iPS will provide a means to generate autologous donor cells for transplantation therapies once the safety issue is overcome.