The Comparison of The Effects of Silybin and Silybin-Phosphatidylcholine on Viability and ESR Expression in Human Breast Cancer T47D Cell Line


Narges Mahmoodi, M.Sc, 1,2Nasrin Motamed, Ph.D, 1,2,*Seyed Hassan Paylakhi, Ph.D, 3
Department of Cell and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran
School of Biology, University College of Science, University of Tehran, Tehran, Iran
School of Biology, Damghan University, Damghan, Iran
Department of Cell and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran
School of Biology, University College of Science, University of Tehran, Tehran, Iran
School of Biology, Damghan University, Damghan, Iran
*Corresponding Address: P.O.Box: 14155-6455 School of Biology University College of Science University of Tehran Tehran Iran Email:motamed2@khayam.ut.ac.ir
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Mahmoodi Narges, Motamed Nasrin, Paylakhi Seyed Hassan. The Comparison of The Effects of Silybin and Silybin-Phosphatidylcholine on Viability and ESR Expression in Human Breast Cancer T47D Cell Line. Cell J. 2014; 16(3): 299-308.

Abstract

Objective

Silybin is a polyphenol with anti-oxidant and anti-cancer properties. The poor bioavailability of some polyphenols can be improved by binding to phosphatidylcholine. In recent years, studies have been conducted to evaluate the anti-cancer effect of silybin. We studied the effect of silybin and silybin-phosphatidylcholine on ESR1 and ESR2 gene expression and viability in the T47D breast cancer cell line.

Materials and Methods

In this experimental study, a 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide test (MTT test) was used to determine doses for cell treatment, and the gene expression was analyzed by real-time reverse transcriptase-polymerase chain reaction (real-time RT- PCR).

Results

Significant dose- and time-dependent cell growth inhibitory effects of silybin and silybin-phosphatidylcholine along with ESR1 down-regulation were observed in T47D cells. In contrast to ESR1, the T47D cell line showed negligible ESR2 expression.

Conclusion

This study suggests that silybin and silybin-phosphatidylcholine down-regulate ESR1 in ER+breast cancers. Results also show that in the T47D cell line, silybindown-regulation of ESR1 compared with silybin.