Ultrastructural Study onApoptotic Effects of Myleran inSpermatogenic Cells in Adult MiceTestis


Mohammad Ghasemi F , Soleimani Rad J , Ghanbari A.A* *,

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Introduction: Myleran is known to have testicular toxicity leading to male infertility. Little is known about ultrastructural effects of myleran on male germ cells. In this study, we examined the possible involvement of apoptosis in the induction of germ cells degeneration following treatment with different doses of myleran using transmission electron microscopy (TEM) and light microscopy (LM). Materials and Methods: At the present study 8 weeks old NMRI mice were divided in 5 groups. Control animals were treated with DMSO. However the other animals in the 2nd, 3rd, 4th and 5th groups were treated intraperitoneally with different doses of myleran: 5, 10, 20 and 40 mg/kg respectively. All animals were dissected after35 days and their testis was evaluated for detection of apoptosis using TEM and LM. Results: In all myleran treated groups except in 2nd group, the nucleous of spermatogonia was marginal hyperchromatin with a crescent form. Primary spermatocytes showed cellular shrinkage and vacuolization inside the cytoplasm. Presence of large spaces between adjacent cells was another sign. Few round spermatids were affected and all signs were increased with the increasing of the dose. In LM study spermatogonia numbers were significantly reduced in the 4th and 5th groups (p