Induction Effect of Deprenyl on Transdifferentiation of BMSCs into Neuron and Glial-Like Cells In Vitro (Pages: 15-22)


MT. Ghorbanian *, T. Tiraihi* , SA. Mesbah Namin , Y. Fathollahi ,

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Introduction: There are the evidences to suggest that bone marrow stromal cells (BMSCs) not only differentiate into mesodermal cells, but also adopt the fate of endodermal and ectodermal cell types. BMSCs can be a valuable cell source as an autograph for clinical application involving regeneration of the central nervous system. Bone marrow stromal cells can be expanded rapidly in vitro and have the potential with appropriate treatment, can be differentiated into neuronal, glial-like cell types. The study of induction of deprenyl on transdifferentiation of BMSCs into neuron and glial-like cells in vitro. Materials and Methods: In the study, bone marrow was extracted from the femur and tibia of adult rat, and then bone marrow stromal cells with 5 passages were proliferated and cultured. Bone marrow stromal cells were differentiated using deprenyl 10-8 M to neuronal- and glial-like cells and were assessed with immunocytochemical method. Before and after neuronal induction with deprenyl, were assessed by anti neurofilament 68(KD), 200(KD), GFAP and Oligo antibodies. Results: Differentiation of the cells that induced by deprenyl were positive reaction for neuronal markers. For determination of the percentage of the neuron and glial-like cells, were counted by immunocyochemical method using anti NF 200 and anti GFAP antibodies. Results showed that 82.71% neuronal-like cells and 25.99% astrocyte-like cells of the cells were positive reaction. Conclusion: Hence we concluded that bone marrow stromal cells can be differentiated into neuronal- and glial-like cells with deprenyl 10-8 M as inducer in vitro.