Effect of Adenosine A1 Recertors of Amygdala on Entorhinal Cortex Kindled Seizures in Rat (Pages: 137-143)

A. Amini Komijani *, J. Mirnajafi-Zadeh * , Y Fathollahi , F. Motamedi ,


Introduction: The role of adenosine A1 receptors of the amygdala on entorhinal cortex kindled seizures was investigated. Material and Methods: Animals were kindled by daily electrical stimutation of entorhinal cortex. In the full kindled animals, N6-cyclohexyladenosine (CHA 10, 50, 100, 500óM), an adenosine A1 receptor agonist and 8-cyclopentyl- 1, 3, dimethylxanthine (CPT, 10, 20 óM), an adenosine A1 receptor antagonist, were microinfused into the amygdala. Results: Data showed that CHA, only at a concentration of 500óM, decreaed entorhinal cortex and amygdala after discharge duration, stage 5 duration and seizure duration seizure duration and increased patency to stage 4 at 15 min post drug injection. Intraamygdala injection of CPT showed that it only at a concentration of 20 óM, reduced patency to stage 4. Intraamygdala pretreatment of animals by intraamygdala injection of CPT (10 óM), 5 min before CHA (500 óM), resulted in blockage of anticonvulsant effects of CHA. Conclusion: These results suggest that the amygdala may have a moderate role in seizure propagation from the entorhinal cortex and its adenosine A1 receptor activity has anticovulsant effects on entorhinal cortex kindled seizures.