Introduction: To evaluate the quick Cryopreservation technique on parameters of motility, morphology, and vitality of spermatozoa from normozoospermic ejaculates. Materials and Methods: In this prospective study, a total of 37 normal ejaculates were evaluated according to WHO criteria, then each sample was divided into two aliquets and sample was cryopreserved with quick freezing method. Sperm parameters of count, motility, morphology and vitality were measured following thawing. In addition, the correlation between Motility survival rate and sperm parameters was studied following freezing. Results: The results showed that the rates of count, normal morphology, progressive motility, and percentage of live spermatozoa were significantly reduced following thawing (P<0.001). The mean of sperm progressive survival rate was 35.62±21.72% which was in direct relation with parameters of progressive motility, morphology, and vitality before the freezing (P<0.05). This was not correlated with non- progressive motility and sperm count (P>0.05). Conclusion: In spite of reduction in sperm parameters following freezing, a vast number of spermatozoa preserve their vitality. This condition is important for infertility treatment programs. In addition, normal parameters of progressive motility, vitality, and morphology play a vital role in molility survival rate of spermatozoa.