The Effect of Lactobacillus crispatus and Lactobacillus rhamnosusCulture Supernatants on Expression of Autophagy Genes and HPV E6 and E7 Oncogenes in The HeLa Cell Line

(Pages: 601-607)
Elahe Motevaseli, M.D., Ph.D., 1Rosa Azam, M.Sc, 2Seyed Mohammad Akrami, M.D., Ph.D., 2Mohammadali Mazlomy, Pharm.D., Ph.D., 3Mojtaba Saffari, M.Sc, 2Mohammad Hossein Modarressi, M.D., Ph.D., 2Maryam Daneshvar, M.Sc, 2,*Soudeh Ghafouri-Fard, M.D., Ph.D., 4,*
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
*Corresponding Addresses: P.O.Box: 14176-13151 Department of Medical Genetics Tehran University of Medical Sciences Tehran Iran P.O.Box: 19857-17443 Department of Medical Genetics Shahid Beheshti University of Medical Sciences Tehran Iran Emails:danesh.mary@gmail.com,s.ghafourifard@sbmu.ac.ir
Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Motevaseli Elahe, Azam Rosa, Akrami Seyed Mohammad, Mazlomy Mohammadali, Saffari Mojtaba, Modarressi Mohammad Hossein, Daneshvar Maryam, Ghafouri-Fard Soudeh. The Effect of Lactobacillus crispatus and Lactobacillus rhamnosusCulture Supernatants on Expression of Autophagy Genes and HPV E6 and E7 Oncogenes in The HeLa Cell Line . Cell J. 2016; 17(4): 601-607.

Abstract

Objective

The aim of this study was to clarify the mechanism by which lactobacilli exert their cytotoxic effects on cervical cancer cells. In addition, we aimed to evalu- ate the effect of lactobacilli on the expression of human papilloma virus (HPV) onco- genes.

Materials and Methods

In this experimental study, using quantitative real-time polymer- ase chain reaction (PCR), we analyzed the expression of CASP3 and three autophagy genes [ATG14, BECN1 and alpha 2 catalytic subunit of AMPK (PRKAA2)] along with HPV18 E6 and E7 genes in HeLa cells before and after treatment with Lactobacillus crispatus and Lactobacillus rhamnosus culture supernatants.

Results

The expression of CASP3 and autophagy genes in HeLa cells was de- creased after treatment with lactobacilli culture supernatants. However, this de- crease was not significant for PRKAA2 when compared with controls. In addition, expression of HPV E6 was significantly decreased after treatment with lactobacilli culture supernatants.

Conclusion

Lactobacilli culture supernatants can decrease expression of ATG14 and BECN1 as well as the HPV E6 oncogene. It has been demonstrated that the main changes occurring during cervical carcinogenesis in cell machinery can be reversed by suppression of HPV oncogenes. Therefore, downregulation of HPV E6 by lacto- bacilli may have therapeutic potential for cervical cancer. As the role of autophagy in cancer is complicated, further work is required to clarify the link between downregula- tion of autophagy genes and antiproliferative effects exerted by lactobacilli.