Histological Study of Bone Marrow and Umbilical Cord Stromal Cell Transplantation in Regenerating Rat Peripheral Nerve


Sam Zarbakhsh, Ph.D., 1,*Nasim Goudarzi, M.Sc., 2Maryam Shirmohammadi, M.Sc., 2Manouchehr Safari, Ph.D., 1
Research Center of Nervous System Stem Cells, Department of Anatomy, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
Research Center of Nervous System Stem Cells, Department of Anatomy, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
*Corresponding Address: P.O.Box: 3519899951 Research Center of Nervous System Stem Cells Department of Anatomy Faculty of Medicine Semnan University of Medical Sciences Semnan Iran Email:szarbakhsh@yahoo.com
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Zarbakhsh Sam, Goudarzi Nasim, Shirmohammadi Maryam, Safari Manouchehr. Histological Study of Bone Marrow and Umbilical Cord Stromal Cell Transplantation in Regenerating Rat Peripheral Nerve. Cell J. 2016; 17(4): 668-677.

Abstract

Objective

Bone marrow and umbilical cord stromal cells are multipotential stem cells that have the ability to produce growth factors that play an important role in survival and generation of axons. The goal of this study was to evaluate the effects of the two different mesenchymal stem cells on peripheral nerve regeneration.

Materials and Methods

In this experimental study, a 10 mm segment of the left sciatic nerve of male Wistar rats (250-300 g) was removed with a silicone tube interposed into this nerve gap. Bone marrow stromal cells (BMSCs) and human umbilical cord stromal cells (HUCSCs) were respectively obtained from rat and human. The cells were sepa- rately cultured and transplanted into the nerve gap. The sciatic nerve regeneration was evaluated by immunohistochemistry, and light and electron microscopy. Moreover, histo- morphology of the gastrocnemius muscle was observed.

Results

The nerve regeneration in the BMSCs and HUCSCs groups that had received the stem cells was significantly more favorable than the control group. In addition, the BM- SCs group was significantly more favorable than the HUCSCs group (P<0.05).

Conclusion

The results of this study suggest that both homograft BMSCs and het- erograft HUCSCs may have the potential to regenerate peripheral nerve injury and transplantation of BMSCs may be more effective than HUCSCs in rat.