Human Neutrophil Elastase Induce Interleukin-10
Expression in Peripheral Blood Mononuclear Cells
through Protein Kinase C Theta/Delta and
Neutrophils have an important role in the rapid innate immune response, and the release or active secretion of elastase from neutrophils is linked to various inflammatory responses. Purpose of this study was to determine how the human neutrophil elastase affects the interleukin-10 (IL-10) response in peripheral blood mononuclear cells (PBMC).
Materials and Methods
In this prospective study, changes in IL-10 messenger RNA (mRNA) and protein expression levels in monocytes derived from human PBMCs were investigated after stimulation with human neutrophil elastase (HNE). A set of inhibitors was used for examining the pathways for IL-10 production induced by HNE.
Reverse transcription polymerase chain reaction (RT-PCR) showed that
stimulation with HNE upregulated
These results indicate that HNE mainly upregulates