Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite

(Pages: 429-437)
Shahnaz Razavi, Ph.D, 1,*Saeed Karbasi, Ph.D, 2Mohammad Morshed, Ph.D, 3Hamid Zarkesh Esfahani, Ph.D, 4Mohammad Golozar, Ph.D, 5Sedigheh Vaezifar, Ph.D, 1,5
Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Medical Physics and Biomedical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Textile Engineering, Isfahan University of Technology, Isfahan, Iran
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran
Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Medical Physics and Biomedical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Textile Engineering, Isfahan University of Technology, Isfahan, Iran
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran
*Corresponding Address: P.O.Box: 81744-176 Department of Anatomical Sciences and Molecular Biology School of Medicine Isfahan University of Medical Sciences Isfahan Iran Email:razavi@med.mui.ac.ir
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Razavi Shahnaz, Karbasi Saeed, Morshed Mohammad, Zarkesh Esfahani Hamid, Golozar Mohammad, Vaezifar Sedigheh. Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite . Cell J. 2015; 17(3): 429-437.

Abstract

Objective

In this study, nano-biocomposite composed of poly (lactide-co-glycolide) (PLGA) and chitosan (CS) were electrospun through a single nozzle by dispersing the CS nano-powders in PLGA solution. The cellular behavior of human adipose derived stem cells (h-ADSCs) on random and aligned scaffolds was then evaluated.

Materials and Methods

In this experimental study, the PLGA/CS scaffolds were prepared at the different ratios of 90/10, 80/20, and 70/30 (w/w) %. Morphology, cell adhesion and prolif- eration rate of h-ADSCs on the scaffolds were assessed using scanning electron microscope (SEM), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and trypan blue staining respectively.

Results

H-ADSCs seeded on the matrices indicated that the PLGA/CS composite matrix with aligned nanofibres and higher content of CS nano-powders gave significantly better performance than others in terms of cell adhesion and proliferation rate (P<0.05).

Conclusion

We found that CS enhanced cell adhesion and proliferation rate, and aligned nanofibers guided cell growth along the longitudinal axis of the nanofibers, which would provide a beneficial approach for tissue engineering.