Inhibitory Effect of Mesenchymal Stem Cell Co-Culture on Erythroid Differentiation of K562 Cells Compared to The Control Group

(Pages: 127-136)
Mahshid Saleh, M.Sc, 1Karim Shamsasanjan, Ph.D, 1Ali Akbari Movassaghpour, Ph.D, 1Parvin Akbarzadehlaleh, Ph.D, 2,*Zahra Molaeipour, M.Sc, 1
Hematology Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Pharmacutical Biotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
Hematology Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Pharmacutical Biotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
* Corresponding Address: P.O.Box: 51664-14766 Department of Pharmacutical Biotechnology Tabriz University of Medical Sciences Tabriz Iran Email:Akbarzadehp@tbzmed.ac.ir
Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Saleh Mahshid, Shamsasanjan Karim, Movassaghpour Ali Akbari, Akbarzadehlaleh Parvin, Molaeipour Zahra. Inhibitory Effect of Mesenchymal Stem Cell Co-Culture on Erythroid Differentiation of K562 Cells Compared to The Control Group . Cell J. 2017; 19(1): 127-136.

Abstract

Objective

Bone marrow mesenchymal stem cells (BMMSCs) reside in the bone marrow and control the process of hematopoiesis. They are an excellent instrument for regenerative treatment and co-culture with hematopoietic stem cells (HSCs).

Materials and Methods

In this experimental study, K562 cell lines were either treated with butyric acid and co-cultured with MSCs, or cultivated in a conditioned medium from MSCs plus butyric acid for erythroid differentiation. We used the trypan blue dye exclusion assay to determine cell counts and viability in each group. For each group, we separately assessed erythroid differentiation of the K562 cell line with Giemsa stain under light microscopy, expression of specific markers of erythroid cells by flowcytometry, and erythroidspecific gene expressions by real-time polymerase chain reaction (RT-PCR).

Results

There was enhandced erythroid differentiation of K562 cells with butyric acid compared to the K562 cell line co-cultured with MSCs and butyric acid. Erythroid differentiation of the K562 cell line cultivated in conditioned medium with butyric acid was higher than the K562 cell line co-cultured with MSCs and butyric acid, but less than K562 cell line treated with butyric acid only.

Conclusion

Our results showed that MSCs significantly suppressed erythropoiesis. Therefore, MSCs would not be a suitable optimal treatment strategy for patients with erythroid leukemia.