Dual Anti-Metastatic and Anti-Proliferative Activity
Assessment of Two Probiotics on HeLa and
HT-29 Cell Lines
Lactobacilli are a group of probiotics with beneficial effects on prevention of
cancer. However, there is scant data in relation with the impacts of probiotics in late-stage
cancer progration, especially metastasis. The present original work was aimed to evaluate
the anti-metastatic and anti-proliferative activity of
Materials and Methods
In this experimental study, the anti-proliferative activities of LRS
and LCS were determined through MTT assay. MRC-5 was used as a normal cell line.
Expression analysis of
Supernatants of these two lactobacilli had cytotoxic effect on HeLa, however
LRS treatment was only effective on HT-29 cell line. In addition, LRS had no side-effect on
normal cells. It was shown that
Herein, we have demonstrated two probiotics which have anti-metastatic effects on malignant cells and they can be administrated to postpone late-stage of cancer disease. LRS and LCS are effective on HeLa cell lines while only the effect of LRS is significant on HT-29, through cytotoxic and anti-metastatic mechanisms. Further assessments are required to evaluate our results on the other cancer cell lines, in advance to use these probiotics in other extensive trial studies.