In Vivo Vascularization of Endothelial Cells Derived from Bone Marrow Mesenchymal Stem Cells in SCID Mouse Model


Abdolamir Allameh, Ph.D, 1,*Maryam Jazayeri, Ph.D, 1,2Maryam Adelipour, M.Sc, 1
Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Department of Biochemistry, School of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Department of Biochemistry, School of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
*Corresponding Address: P.O.Box: 14115-111 Department of Clinical Biochemistry Faculty of Medical Sciences Tarbiat Modares University Tehran Iran Email:allameha@modares.ac.ir
Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Allameh Abdolamir, Jazayeri Maryam, Adelipour Maryam. In Vivo Vascularization of Endothelial Cells Derived from Bone Marrow Mesenchymal Stem Cells in SCID Mouse Model. Cell J. 2016; 18(2): 179-188.

Abstract

Objective

In vivo and in vitro stem cell differentiation into endothelial cells is a promising area of research for tissue engineering and cell therapy.

Materials and Methods

We induced human mesenchymal stem cells (MSCs) to differentiate to endothelial cells that had the ability to form capillaries on an extracellular matrix (ECM) gel. Thereafter, the differentiated endothelial cells at early stage were characterized by expression of specific markers such as von Willebrand factor (vWF), vascular endothelial growth factor (VEGF) receptor 2, and CD31. In this experimental model, the endothelial cells were transplanted into the groins of severe combined immunodeficiency (SCID) mice. After 30 days, we obtained tissue biopsies from the transplantation sites. Biopsies were processed for histopathological and double immunohistochemistry (DIHC) staining.

Results

Endothelial cells at the early stage of differentiation expressed endothelial markers. Hematoxylin and eosin (H&E) staining, in addition to DIHC demonstrated homing of the endothelial cells that underwent vascularization in the injected site.

Conclusion

The data clearly showed that endothelial cells at the early stage of differentiation underwent neovascularization in vivo in SCID mice. Endothelial cells at their early stage of differentiation have been proven to be efficient for treatment of diseases with impaired vasculogenesis.