The Evaluation of Nerve Growth Factor Over Expression
on Neural Lineage Specific Genes in Human
Mesenchymal Stem Cells
Treatment and repair of neurodegenerative diseases such as brain tumors,
spinal cord injuries, and functional disorders, including Alzheimer’s disease, are challenging problems. A common treatment approach for such disorders involves the use of
mesenchymal stem cells (MSCs) as an alternative cell source to replace injured cells.
However, use of these cells in hosts may potentially cause adverse outcomes such as tumorigenesis and uncontrolled differentiation. In attempt to generate mesenchymal derived
neural cells, we have infected MSCs with recombinant lentiviruses that expressed nerve
growth factor (
Materials and Methods
In this experimental study, we cloned the
We observed neural morphological changes after three days. Quantitative PCR
showed that expressions of NESTIN, glial derived neurotrophic factor (
Ectopic expression of NGF can induce neurogenesis in MSCs. Direct injection of MSCs may cause tumorigenesis and an undesirable outcome. Therefore an alternative choice to overcome this obstacle may be the utilization of differentiated neural stem cells.