Overexpression of Annexin A1 Suppresses Pro-Inflammatory
Factors in PC12 Cells Induced by
Annexin A1 (ANXA1) is suggested to have anti-inflammatory function. However, the precise function of ANXA1 has remained unclear. In this study, we therefore examined the potency of ANXA1 in regulating reactive oxygen species (ROS) production and suppressing pro-inflammatory responses in PC12 cells induced by 1-methyl-4-phenylpyridinium (MPP+).
Materials and Methods
In this experimental study, cDNA of ANXA1 was cloned and
inserted to the PGL268 pEpi-FGM18F vector to produce a recombinant PGL/ANXA1 vector for transfection into the PC12 cells. ANXA1 transfected cells were then treated with
MPP+. Apoptosis and the content of pro-inflammatory factors including ROS, Interlukin-6
Data revealed that overexpression of ANXA1 is associated with decreased levels of ROS and expression level of
ANXA1 may be considered as an agent for prevention of neurodegenerative or inflammatory conditions.