Overexpression of Annexin A1 Suppresses Pro-Inflammatory Factors in PC12 Cells Induced by 1-Methyl-4-Phenylpyridinium


Abbas Kiani-Esfahani, M.Sc, 1Sedigheh Kazemi Sheykhshabani, M.Sc, 2Maryam Peymani, Ph.D, 1,3Motahare-Sadat Hashemi, M.Sc, 1Kamran Ghaedi, Ph.D, 1,4,*Mohammad Hossein Nasr-Esfahani, Ph.D, 1,*
Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Sahrekord, Iran
Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran
Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Sahrekord, Iran
Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran
*Corresponding Address: P.O.Box: 816513-1378 Department of Cellular Biotechnology Cell Science Research Center Royan Institute for Biotechnology ACECR Isfahan Iran Emails:kamranghaedi@royaninstitute.org,mh_nasr@royaninstitute.org
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Kiani-Esfahani Abbas, Kazemi Sheykhshabani Sedigheh, Peymani Maryam, Hashemi Motahare-Sadat, Ghaedi Kamran, Nasr-Esfahani Mohammad Hossein. Overexpression of Annexin A1 Suppresses Pro-Inflammatory Factors in PC12 Cells Induced by 1-Methyl-4-Phenylpyridinium . Cell J. 2016; 18(2): 197-204.

Abstract

Objective

Annexin A1 (ANXA1) is suggested to have anti-inflammatory function. However, the precise function of ANXA1 has remained unclear. In this study, we therefore examined the potency of ANXA1 in regulating reactive oxygen species (ROS) production and suppressing pro-inflammatory responses in PC12 cells induced by 1-methyl-4-phenylpyridinium (MPP+).

Materials and Methods

In this experimental study, cDNA of ANXA1 was cloned and inserted to the PGL268 pEpi-FGM18F vector to produce a recombinant PGL/ANXA1 vector for transfection into the PC12 cells. ANXA1 transfected cells were then treated with MPP+. Apoptosis and the content of pro-inflammatory factors including ROS, Interlukin-6 (IL-6), inducible nitric oxide synthase (iNOS) and nuclear factor-kappa B (NF-κB) were assessed by flow-cytometry, real-time quantitative polymerase chain reaction (RT-qPCR) and western blot in ANXA1-transfected cells and the data were compared with those obtained from mock and control cells.

Results

Data revealed that overexpression of ANXA1 is associated with decreased levels of ROS and expression level of IL-6 and iNOS transcripts, and NF-κB protein in MPP+ treated PC12 cells.

Conclusion

ANXA1 may be considered as an agent for prevention of neurodegenerative or inflammatory conditions.