Differential Incorporation of β-actin as A Component of
RNA Polymerase II into Regulatory Regions of
Stemness/Differentiation Genes in Retinoic
Acid-Induced Differentiated Human
Embryonic Carcinoma Cells
Nuclear actin is involved in transcription regulation by recruitment of histone
modifiers and chromatin remodelers to the regulatory regions of active genes. In recent
years, further attention has been focused on the role of actin as a nuclear protein in
transcriptional processes. In the current study, the epigenetic role of nuclear actin on
transcription regulation of two stemness (
Materials and Methods
In this experimental study, differentiation of embryonal cells was induced by retinoic acid (RA), and quantitative real-time polymerase chain reaction (PCR) was used to evaluate differential expression of marker genes before and 3 days after RA- induced differentiation. Chromatin immunoprecipitation (ChIP) coupled with real-time PCR was then undertaken to monitor the incorporation of β-actin, as a functional component of RNA polymerase II, in the regulatory regions of marker genes.
Data showed significant change in nuclear actin incorporation into the promoter
We emphasize the dynamic functional role of nuclear actin in differentiation of embryonal cells and its role as a subunit of RNA polymerase II.