Peroxisome Proliferator-Activated Receptor γ
Activity is Required for Appropriate
Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the
PPAR nuclear receptor superfamily. Although PPARγ acts as a master transcription factor
in adipocyte differentiation, it is also associated with a variety of cell functions including
carbohydrate and lipid metabolism, glucose homeostasis, cell proliferation and cell differentiation. This study aimed to assess the expression level of
Materials and Methods
In this an intervening study, mESCs were subjected to cardiac differentiation. Total RNA was extracted from the cells and quantitative real time polymerase chain reaction (qPCR) was carried out to estimate level of gene expression. Furthermore, the requirement of PPARγ in cardiac differentiation of mESCs, during cardiac progenitor cells (CPCs) formation, was examined by applying the respective agonist and antagonist.
The obtained data revealed an elevation in the expression level of
We conclude that PPARγ modulation has an effective role on cardiac differentiation of mESCs at the early stage of cardiomyogenesis.